What is the difference between Gram-positive and Gram-negative bacteria? Gram-positive bacteria show blue or purple after gram-staining in a laboratory test. They have thick cell walls. Gram-negative bacteria show pink or red on staining and have thin walls.
Gram positive bacteria possess a thick (20–80 nm) cell wall as outer shell of the cell. In contrast Gram negative bacteria have a relatively thin (<10 nm) layer of cell wall, but harbour an additional outer membrane with several pores and appendices.
Actinobacteria is the taxonomic name of the class of high G+C gram-positive bacteria. This class includes the genera Actinomyces, Arthrobacter, Corynebacterium, Frankia, Gardnerella, Micrococcus, Mycobacterium, Nocardia, Propionibacterium, Rhodococcus, and Streptomyces.
coli, Salmonella, Shigella, and other Enterobacteriaceae, Pseudomonas, Moraxella, Helicobacter, Stenotrophomonas, Bdellovibrio, acetic acid bacteria, Legionella etc. Other notable groups of gram-negative bacteria include the cyanobacteria, spirochaetes, green sulfur, and green non-sulfur bacteria.
Gram-positive bacteria are species of bacteria that have a thick peptidoglycan outer layer that absorbs antibiotics easily, making them easier to kill. Gram-negative bacteria, on the other hand, have a thin peptidoglycan layer that is covered by multiple thin layers of membrane that can better-resist antibiotics.
Escherichia coli (E. coli) is a Gram-negative, rod-shaped, facultative anaerobic bacterium.
Gram-negative bacteria are more harmful than gram-positive bacteria. This is because gram-negative bacteria have a hard, protective outer shell, which makes them more resistant to antibiotics and hence harder to kill.
Gram-Negative cocci: Neisseria, Branhamella, Moraxella, Acinetobacter, Veillonella.
The organisms are identified based on color and shape. Gram-positive organisms are either purple or blue in color, while gram-negative organisms are either pink or red in color. Bacilli are rod-shaped, while cocci are spherical.
A mixture of hydrogen peroxide and ascorbic acid has been found to generate an antibacterial mechanism which is active against gram-negative bacteria. It results in bacterial death and renders the organism sensitive to lysis by lysozyme.
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